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Microbiology · USMLE Step 1 · Healthcare and Nursing

USMLE Microbiology: Bacterial Genetics and Growth

Bacterial genetic transfer mechanisms, plasmids, growth curve phases, and biofilm formation, one concept per card.

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34 basic cards for USMLE Step 1 Microbiology. Set 3 of 12 in USMLE Microbiology. It opens with "What is bacterial transformation?" and "What is bacterial competence?". Study it alongside the other USMLE Step 1 sets below.

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What is bacterial transformation?
Show answerUptake of naked (free) DNA from the environment by a bacterial cell.
What is bacterial competence?
Show answerThe physiological state of being able to take up extracellular DNA.
Which classic experiment first demonstrated bacterial transformation?
Show answerGriffith's experiment with Streptococcus pneumoniae strains.
What did the Avery-MacLeod-McCarty experiment identify?
Show answerDNA as the molecule responsible for bacterial transformation.
What is generalized transduction?
Show answerA lytic phage mistakenly packages a random fragment of host bacterial DNA into a phage capsid and transfers it to another bacterium.
What is specialized transduction?
Show answerA lysogenic phage excises imprecisely from the host chromosome, carrying adjacent bacterial genes to the next bacterium it infects.
What is lysogenic conversion?
Show answerA change in a bacterium's phenotype caused by genes carried by a prophage integrated into its genome.
Which toxin's gene is delivered to Corynebacterium diphtheriae by a lysogenic phage?
Show answerDiphtheria toxin.
Which toxin's gene is delivered to Vibrio cholerae by a lysogenic phage?
Show answerCholera toxin.
Which toxin's gene is delivered to E. coli O157:H7 by a lysogenic phage?
Show answerShiga toxin.
What is bacterial conjugation?
Show answerDirect transfer of DNA from a donor to a recipient bacterium through cell-to-cell contact via a pilus.
What is transferred during F+ x F- conjugation?
Show answerOnly the F plasmid; the recipient becomes F+.
What is transferred during Hfr x F- conjugation?
Show answerThe F plasmid plus adjacent chromosomal genes; the recipient rarely becomes F+.
What structure allows an F+ or Hfr bacterium to attach to a recipient for conjugation?
Show answerThe sex pilus.
What is a transposon?
Show answerA mobile genetic element that can move between different locations in DNA, including between a plasmid and a chromosome.

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