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Microbiology · USMLE Step 1

USMLE Microbiology: Bacterial Genetics and Growth

Bacterial genetic transfer mechanisms, plasmids, growth curve phases, and biofilm formation, one concept per card.

34 cards · basic cards · AI-written, checked twice. Edit anything.

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What is bacterial transformation?
Uptake of naked (free) DNA from the environment by a bacterial cell.
What is bacterial competence?
The physiological state of being able to take up extracellular DNA.
Which classic experiment first demonstrated bacterial transformation?
Griffith's experiment with Streptococcus pneumoniae strains.
What did the Avery-MacLeod-McCarty experiment identify?
DNA as the molecule responsible for bacterial transformation.
What is generalized transduction?
A lytic phage mistakenly packages a random fragment of host bacterial DNA into a phage capsid and transfers it to another bacterium.
What is specialized transduction?
A lysogenic phage excises imprecisely from the host chromosome, carrying adjacent bacterial genes to the next bacterium it infects.
What is lysogenic conversion?
A change in a bacterium's phenotype caused by genes carried by a prophage integrated into its genome.
Which toxin's gene is delivered to Corynebacterium diphtheriae by a lysogenic phage?
Diphtheria toxin.
Which toxin's gene is delivered to Vibrio cholerae by a lysogenic phage?
Cholera toxin.
Which toxin's gene is delivered to E. coli O157:H7 by a lysogenic phage?
Shiga toxin.
What is bacterial conjugation?
Direct transfer of DNA from a donor to a recipient bacterium through cell-to-cell contact via a pilus.
What is transferred during F+ x F- conjugation?
Only the F plasmid; the recipient becomes F+.
What is transferred during Hfr x F- conjugation?
The F plasmid plus adjacent chromosomal genes; the recipient rarely becomes F+.
What structure allows an F+ or Hfr bacterium to attach to a recipient for conjugation?
The sex pilus.
What is a transposon?
A mobile genetic element that can move between different locations in DNA, including between a plasmid and a chromosome.

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