Culture media, staining techniques, and molecular diagnostic methods (PCR, serology) used to identify microorganisms, one technique per card.
40 cards · basic cards · AI-written, checked twice. Edit anything.
- What does the Gram stain differentiate bacteria based on?
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The thickness of their peptidoglycan cell wall layer.
- What color do gram-positive bacteria appear after Gram staining?
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Purple (violet).
- What color do gram-negative bacteria appear after Gram staining?
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Pink (red).
- Which stain is used to identify Mycobacterium species?
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Ziehl-Neelsen (acid-fast) stain.
- What cell wall component makes Mycobacterium resistant to acid decolorization?
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Mycolic acid.
- What staining method uses fluorescence for rapid screening of acid-fast bacilli?
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Auramine-rhodamine stain.
- Which stain visualizes the polysaccharide capsule of Cryptococcus neoformans?
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India ink stain.
- Which stain is used to detect blood parasites like Plasmodium and Borrelia?
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Giemsa stain.
- Which stain identifies Pneumocystis jirovecii cysts in lung tissue?
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Methenamine silver stain (GMS).
- Which stain is used to diagnose Whipple disease?
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Periodic acid-Schiff (PAS) stain.
- Which stain highlights fungal cell walls under fluorescence microscopy?
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Calcofluor white stain.
- Which stool stain is used to identify ova and parasites?
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Trichrome stain.
- What finding on a Tzanck smear suggests herpesvirus infection?
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Multinucleated giant cells.
- What is MacConkey agar used to differentiate?
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Lactose-fermenting from non-lactose-fermenting gram-negative bacteria.
- What color does E. coli produce on eosin methylene blue (EMB) agar?
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A green metallic sheen.