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Microbiology · USMLE Step 1 · Healthcare and Nursing

USMLE Microbiology: Lab Diagnostic Techniques

Culture media, staining techniques, and molecular diagnostic methods (PCR, serology) used to identify microorganisms, one technique per card.

About this set

40 basic cards for USMLE Step 1 Microbiology. Set 8 of 12 in USMLE Microbiology. It opens with "What does the Gram stain differentiate bacteria based on?" and "What color do gram-positive bacteria appear after Gram staining?". Study it alongside the other USMLE Step 1 sets below.

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What does the Gram stain differentiate bacteria based on?
Show answerThe thickness of their peptidoglycan cell wall layer.
What color do gram-positive bacteria appear after Gram staining?
Show answerPurple (violet).
What color do gram-negative bacteria appear after Gram staining?
Show answerPink (red).
Which stain is used to identify Mycobacterium species?
Show answerZiehl-Neelsen (acid-fast) stain.
What cell wall component makes Mycobacterium resistant to acid decolorization?
Show answerMycolic acid.
What staining method uses fluorescence for rapid screening of acid-fast bacilli?
Show answerAuramine-rhodamine stain.
Which stain visualizes the polysaccharide capsule of Cryptococcus neoformans?
Show answerIndia ink stain.
Which stain is used to detect blood parasites like Plasmodium and Borrelia?
Show answerGiemsa stain.
Which stain identifies Pneumocystis jirovecii cysts in lung tissue?
Show answerMethenamine silver stain (GMS).
Which stain is used to diagnose Whipple disease?
Show answerPeriodic acid-Schiff (PAS) stain.
Which stain highlights fungal cell walls under fluorescence microscopy?
Show answerCalcofluor white stain.
Which stool stain is used to identify ova and parasites?
Show answerTrichrome stain.
What finding on a Tzanck smear suggests herpesvirus infection?
Show answerMultinucleated giant cells.
What is MacConkey agar used to differentiate?
Show answerLactose-fermenting from non-lactose-fermenting gram-negative bacteria.
What color does E. coli produce on eosin methylene blue (EMB) agar?
Show answerA green metallic sheen.

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