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Microbiology · USMLE Step 1

USMLE Microbiology: Lab Diagnostic Techniques

Culture media, staining techniques, and molecular diagnostic methods (PCR, serology) used to identify microorganisms, one technique per card.

40 cards · basic cards · AI-written, checked twice. Edit anything.

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What does the Gram stain differentiate bacteria based on?
The thickness of their peptidoglycan cell wall layer.
What color do gram-positive bacteria appear after Gram staining?
Purple (violet).
What color do gram-negative bacteria appear after Gram staining?
Pink (red).
Which stain is used to identify Mycobacterium species?
Ziehl-Neelsen (acid-fast) stain.
What cell wall component makes Mycobacterium resistant to acid decolorization?
Mycolic acid.
What staining method uses fluorescence for rapid screening of acid-fast bacilli?
Auramine-rhodamine stain.
Which stain visualizes the polysaccharide capsule of Cryptococcus neoformans?
India ink stain.
Which stain is used to detect blood parasites like Plasmodium and Borrelia?
Giemsa stain.
Which stain identifies Pneumocystis jirovecii cysts in lung tissue?
Methenamine silver stain (GMS).
Which stain is used to diagnose Whipple disease?
Periodic acid-Schiff (PAS) stain.
Which stain highlights fungal cell walls under fluorescence microscopy?
Calcofluor white stain.
Which stool stain is used to identify ova and parasites?
Trichrome stain.
What finding on a Tzanck smear suggests herpesvirus infection?
Multinucleated giant cells.
What is MacConkey agar used to differentiate?
Lactose-fermenting from non-lactose-fermenting gram-negative bacteria.
What color does E. coli produce on eosin methylene blue (EMB) agar?
A green metallic sheen.

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