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Allied Health · Phlebotomy · Healthcare and Nursing

Phlebotomy: Order of Draw and Additives

Blood collection tube colors, their additives, and the correct order of draw tested on phlebotomy certification exams.

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33 basic cards for Phlebotomy Allied Health. Set 3 of 3 in Phlebotomy. It opens with "What is the correct first tube to draw in the standard order of draw?" and "What does SPS stand for in a blood culture tube?". More Healthcare and Nursing decks are listed below.

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What is the correct first tube to draw in the standard order of draw?
Show answerBlood culture tube (yellow top, SPS)
What does SPS stand for in a blood culture tube?
Show answerSodium polyanetholesulfonate
Which tube color is drawn second in the order of draw, right after blood cultures?
Show answerLight blue top
Light blue top tube additive
Show answerSodium citrate
What test is the light blue (sodium citrate) tube used for?
Show answerCoagulation studies such as PT and PTT
Why must a light blue coagulation tube be filled to its exact draw volume?
Show answerTo keep the correct blood-to-citrate ratio for accurate results
What color is a plain serum tube with no additive and no gel?
Show answerRed top
What is the common abbreviation for a gold or tiger-top serum tube?
Show answerSST (serum separator tube)
What does the gel in an SST (gold top) tube do?
Show answerSeparates serum from blood cells during centrifugation
What additive is in a gold/tiger top (SST) tube besides the separator gel?
Show answerClot activator
In the order of draw, do serum tubes (red/SST) come before or after coagulation tubes?
Show answerAfter
What additive is found in a green top tube?
Show answerHeparin (sodium or lithium)
What is a green top (heparin) tube commonly used for?
Show answerPlasma chemistry tests, such as stat electrolytes or ammonia
How does heparin prevent blood from clotting?
Show answerIt inhibits thrombin formation
Lavender (purple) top tube additive
Show answerEDTA

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