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Phlebotomy · Phlebotomy Certification

Phlebotomy: Non-Blood Specimen Collection

Collection procedures for urine, throat, and other non-blood specimens a phlebotomist may be asked to obtain.

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What is the correct patient position for obtaining a throat culture?
Seated or standing with the head tilted back and mouth wide open, tongue depressed.
How deep should a throat culture swab be inserted?
To the back of the throat without touching the teeth, lips, or tongue.
What type of container is required for cerebrospinal fluid collection?
A sterile, plain tube with no additives.
What is the standard volume of urine required for a routine urinalysis?
30 to 50 milliliters.
Describe the first step in a clean-catch midstream urine collection for females.
Spread the labia with one hand and wipe from front to back with a sanitizing wipe.
What should a male patient do before providing a clean-catch midstream urine specimen?
Retract the foreskin if uncircumcised, and clean the urethral opening with a sanitizing wipe.
How should the patient discard urine at the start of a clean-catch collection?
The first portion of the stream is discarded into the toilet, then the midstream portion is collected.
What container type is used for a 24-hour urine collection?
A large plastic jug with a preservative already added, usually provided by the laboratory.
Where should a 24-hour urine collection jug be stored during collection?
In the refrigerator or on ice at 2 to 8 degrees Celsius.
What container type is required for sputum collection?
A sterile, leak-proof container with a tight-fitting lid.
When should sputum be collected from a patient?
First thing in the morning, before eating or drinking, when sputum has accumulated overnight.
What is the minimum acceptable volume of sputum for a laboratory culture?
One to two milliliters.
What instructions should you give a patient to ensure they provide sputum rather than saliva?
Instruct them to cough deeply from the lungs, not just spit from the mouth.
What container is appropriate for stool specimen collection?
A sterile plastic container with a tight-fitting lid, often with a collection spoon or spatula attached.
How much stool specimen is typically needed for laboratory testing?
One to two teaspoons or about 5 to 10 grams.

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