UV-Vis, IR, NMR, mass spectrometry, atomic absorption, GC, HPLC and electrophoresis.
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- What is the wavelength range of UV-Vis spectroscopy?
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200-800 nm- State the Beer-Lambert Law
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A = εbc, where absorbance equals molar absorptivity times path length times concentration- Define molar absorptivity
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The ability of a substance to absorb light at a specific wavelength, with units M^-1 cm^-1- What causes electronic transitions in UV-Vis spectroscopy?
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Absorption of photons causing electrons to jump from lower to higher energy levels- What is a chromophore?
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A group of atoms that absorbs light and is responsible for color in a molecule- What wavelength range does IR spectroscopy cover?
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2,500-25,000 nm or 4,000-400 cm^-1 in wavenumbers- What does IR spectroscopy measure?
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Absorption of infrared radiation by molecular vibrations and rotations- At what wavenumber does a typical C=O stretch appear in IR?
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Around 1,700 cm^-1- What is the fingerprint region in IR spectroscopy?
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The region below 1,500 cm^-1 that is unique to each compound- What does an O-H stretch appear as in an IR spectrum?
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A broad absorption band around 3,200-3,600 cm^-1- What does chemical shift measure in NMR?
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The position of a nuclear signal relative to a reference standard, reported in ppm- What is spin-spin coupling in NMR?
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The indirect interaction between nuclear spins through bonding electrons- State the n+1 rule for NMR splitting
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A nucleus with n neighboring equivalent nuclei appears as a multiplet with n+1 lines- What is the key difference between 1H and 13C NMR?
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1H NMR detects hydrogen nuclei while 13C NMR detects carbon-13 nuclei- What does ppm stand for in NMR?
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Parts per million
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