PCR, gel electrophoresis, cloning, CRISPR, sequencing, blotting, recombinant DNA and model organism tools.
About this set
39 basic cards for College Biology. Set 2 of 2 in Molecular Biology. It opens with "What does PCR stand for?" and "What are the three main steps of each PCR cycle, in order?". Study it alongside the other College sets below.
39 cards · basic cards · AI-written, checked twice. Edit anything.
- What does PCR stand for?
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Polymerase Chain Reaction- What are the three main steps of each PCR cycle, in order?
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Denaturation, annealing, extension- What is the typical denaturation temperature in PCR?
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94-95 degrees Celsius- What is the typical annealing temperature in PCR?
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50-65 degrees Celsius- What is the typical extension temperature in PCR?
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72 degrees Celsius- What DNA polymerase is typically used in PCR?
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Taq polymerase- What is the function of primers in PCR?
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To bind to the template DNA and provide a starting point for DNA synthesis- Approximately how many copies of DNA result from 30 cycles of PCR?
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About 1 billion copies (2 to the 30th power)- What is gel electrophoresis?
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A laboratory technique that separates DNA or proteins based on size and charge using an electric field- Why does DNA migrate through a gel when an electric field is applied?
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DNA has a negatively charged phosphate backbone, so it moves toward the positive electrode- In gel electrophoresis, what determines how far DNA fragments travel?
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Their size; smaller fragments move farther than larger fragments- What is agarose used for in gel electrophoresis?
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As the gel matrix to provide pores through which DNA fragments migrate- How does ethidium bromide allow visualization of DNA?
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It intercalates between DNA base pairs and fluoresces when exposed to UV light- What is molecular cloning?
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The process of creating copies of a specific DNA sequence and inserting it into a vector or host cell- What is a restriction enzyme?
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A protein that cuts DNA at specific recognition sequences
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